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You are here:Home » Antibodies » Antibodies-Transport Proteins » Anti -Dopamine Transporter, CT (DAT)

Anti -Dopamine Transporter, CT (DAT)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
DAT is principally expressed in the presynaptic membrane of dopaminergic nerve terminals, where it mediates the rapid removal of dopamine from the synaptic cleft after neuronal stimulation. As such, DAT plays an important role in regulating the availability of dopamine in the synaptic cleft and thus in modulating the physiological functions of dopamine.
The reuptake of dopamine by DAT is inhibited by psychostimulants such as cocaine and amphetamine.
Catalog #D9020-30A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry (frozen). Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:1000
Western Blot: 1:1000,detects a band of approximately 88kD in human caudate cell lysates
Optimal dilution determined by the researcher.
Recommended Secondary ReagentsSheep anti rabbit IgG:HRP
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 0.1% BSA, 0.09% BSA and 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to a region within the C-terminus of human dopamine transporter.
SpecificitySpecific for human dopamine transporter (DAT), also known as solute carrier family 6.
Species Cross ReactivityMonkey
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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