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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH

DAT is principally expressed in the presynaptic membrane of dopaminergic nerve terminals, where it mediates the rapid removal of dopamine from the synaptic cleft after neuronal stimulation. As such, DAT plays an important role in regulating the availability of dopamine in the synaptic cleft and thus in modulating the physiological functions of dopamine.
The reuptake of dopamine by DAT is inhibited by psychostimulants such as cocaine and amphetamine.
Catalog #	D9020-30A
Applications	Suitable for use in Western Blot and Immunohistochemistry (frozen). Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:1000
	Western Blot: 1:1000,detects a band of approximately 88kD in human caudate cell lysates
	Optimal dilution determined by the researcher.
Recommended Secondary Reagents	Sheep anti rabbit IgG:HRP
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Form	Supplied as a liquid in 10mM HEPES, pH 7.5, 0.1% BSA, 0.09% BSA and 50% glycerol.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide corresponding to a region within the C-terminus of human dopamine transporter.
Specificity	Specific for human dopamine transporter (DAT), also known as solute carrier family 6.
Species Cross Reactivity	Monkey
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.