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Anti -DPH1 (Diphthamide Biosynthesis Protein 1, DPH2-like 1, DPH2L1, DPH2L, FLJ33211, Ovarian Cancer Associated Gene 1, OVCA1) (Azide Free)
Anti -DPH1 (Diphthamide Biosynthesis Protein 1, DPH2-like 1, DPH2L1, DPH2L, FLJ33211,
Ovarian Cancer Associated Gene 1, OVCA1) (Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IF |
|
| Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]). | | | Catalog # | D9150-74 | | Applications | Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:500-1:1000 | | ELISA: 1ug/ml-3ng/ml | | Optimal dilutions to be determined by the researcher. | | Hybridoma | Sp2/0 myeloma cells with spleen cells from Balb/c mice. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 10B2757 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant protein corresponding to aa216-325 of human DPH1. | | Specificity | Recognizes human DPH1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Candidate Tumor Suppressor in Ovarian Cancer 1, DPH1 Homolog (S. cerevisiae) |
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