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You are here:Home » Antibodies » Abs to Enzymes, Synthase » Anti -DPM1 (Dolichol-phosphate Mannosyltransferase, Dolichol-phosphate Mannose Synthase, DPM Synthase, Dolichyl-phosphate beta-D-mannosyltransferase, Mannose-P-dolichol Synthase, MPD Synthase)

Anti -DPM1 (Dolichol-phosphate Mannosyltransferase, Dolichol-phosphate Mannose Synthase,
DPM Synthase, Dolichyl-phosphate beta-D-mannosyltransferase, Mannose-P-dolichol Synthase,
MPD Synthase)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2.
Catalog #D9150-78B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 0.1-0.3ug/ml, observed in human liver lysates on ~28kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to RELEVRSPRQNKYS-C, from human DPM1, at N-terminal (NP_003850.1).
SpecificityRecognizes human DPM1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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