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D9370-02 Rabbit Anti-DRAM, NT (Damage-Regulated Autophagy Modulator)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q8N682
Isotype
IgG
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Hu Mo Rt
Gene ID
55332
Shipping Temp
Blue Ice
Storage Temp
-20°C

Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes together promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence Other applications not tested.
Recommended Dilutions
Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 2-2.5ug/ml Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
K562 cell lysate, human liver tissue lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 16aa near the N-terminal of human DRAM. Epitope: located within aa30-80
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DRAM. Species Crossreactivity: mouse and rat.
References
1. Crighton D, Wilkinson S, O'Prey J, et al. Cell 2006; 126:121-34. 2. Gozuacik D and Kimchi A. Oncogene. 2004; 23:2891-906. 3. Crighton D, et al., Autophagy 2007; 3:72-4.
USBio References
No references available
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