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You are here:Home » Antibodies » Antibodies-Neuroscience » Anti -DREAM (Downstream Regulatory Element Antagonist Modulator, Calsenilin)

Anti -DREAM (Downstream Regulatory Element Antagonist Modulator, Calsenilin)


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Clone Host Grade Applications
Monoclonal Mouse Ascites B IP
Catalog #D9450-01
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:10,000 detected Calsenilin/DREAM in RIPA homogenate from human Alzheimer’s diseased brain. Human Alzheimer’s diseased brain homogenate was resolved by electrophoresis, transferred to nitrocellulose and probed with D9450-01(1:10,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 5ul immunoprecipitated Calsenilin/DREAM from 500ug of mouse brain RIPA homogenate.
Optimal dilutions to be determined by the researcher.
Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No4H321
ConcentrationNot determined
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloridel, 0.05% sodium azide before the addition of glycerol to 30%.
ImmunogenFull length GST fusion protein of human Calsenilin/DREAM.
SpecificityRecognizes human Calsenilin/DREAM, Mr ~30kD. A non-specific protein was also detected, Mr ~24kD. Species Crossreactivity: Rat and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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