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Anti -DREAM (Downstream Regulatory Element Antagonist Modulator, Calsenilin)
Anti -DREAM (Downstream Regulatory Element Antagonist Modulator, Calsenilin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
B IP |
|
| Catalog # | D9450-01 | | Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:10,000 detected Calsenilin/DREAM in RIPA homogenate from human Alzheimer’s diseased brain. Human Alzheimer’s diseased brain homogenate was resolved by electrophoresis, transferred to nitrocellulose and probed with D9450-01(1:10,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Immunoprecipitation: 5ul immunoprecipitated Calsenilin/DREAM from 500ug of mouse brain RIPA homogenate. | | Optimal dilutions to be determined by the researcher. | | Recommended secondary antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo | | I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Clone No | 4H321 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloridel, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Ascites | | Immunogen | Full length GST fusion protein of human Calsenilin/DREAM. | | Specificity | Recognizes human Calsenilin/DREAM, Mr ~30kD. A non-specific protein was also detected, Mr ~24kD. Species Crossreactivity: Rat and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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