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Anti -DRIP-130 (Vitamin D Receptor Interacting Protein 130, Sur2, CRSP130, ARC130, NAT140, TRAP150b)
Anti -DRIP-130 (Vitamin D Receptor Interacting Protein 130, Sur2, CRSP130, ARC130, NAT140,
TRAP150b)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Purified |
E B IP |
|
| DRIPs are a distinct set of ligand-dependent proteins that interact with the vitamin D receptor (VDR). Together, these proteins constitute a new cofactor complex. DRIPs bind to several nuclear receptors to mediate ligand-dependent enhancement of transcription by VDR and the thyroid-hormone receptor in cell-free transcription assays. The DRIPs are almost indistinguishable from components of another cofactor complex called ARC, which is recruited by other types of transcription activators to mediate transactivation on chromatin-assembled templates. The role of nuclear-receptor ligands may, in part, be to recruit such a cofactor complex to the receptor and, in doing so, to enhance transcription of target genes. In humans, interaction with Sur-2 is required for transcription to be activated by the activation domain of a transcription factor of the ETS-family in response to activated mitogen-activated protein (MAP) kinase. | | | Catalog # | D9475 | | Applications | Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000. Detects a 130kD band | | Immunoprecipitation: 1:100-1:200. Pre-clearing of the lysate with a non-specific rabbit IgG is helpful to reduce background. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Mouse | | Concentration | ~80mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added. | | Purity | Purified serum | | Immunogen | Synthetic peptide corresponding to mouse DRIP-130 residues 897-916 (based on human DRIP-130 residue numbering) conjugated to KLH. | | Specificity | Recognizes mouse DRIP-130 (vitamin-D-receptor interacting protein 130). Used to detect DRIP130 using nuclear extracts from mouse thymus, spleen and brain. Species Crossreactivity: Human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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