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You are here:Home » Antibodies » Abs to Enzymes, ATPase » Anti -Ductin (Vha16, EP2372, V-ATPase, VHA, Vha1, vha16, vha16-1, VHA16K, Vhac)

Anti -Ductin (Vha16, EP2372, V-ATPase, VHA, Vha1, vha16, vha16-1, VHA16K, Vhac)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IC
Ductin is the major core component of gap junctions and recent structural data shows it to be a four alpha-helical bundle which fits particularly well into a low resolution model of the gap junction channel. Ductin is also the main membrane component of the vacuolar H+-ATPase that is found in all eukaryotes and it seems likely that the gap junction channel first evolved as a housing for the rotating spindle of these proton pumps. Because ductin protrudes little from the membrane, other proteins are required to bring cell surfaces close enough together to form gap junctions. Such proteins may include connexins, a large family of proteins found in vertebrates.
Catalog #D9650
ApplicationsSuitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:500-1:1000
Immunocytochemistry: 1:100-1:500
Immunohistochemistry: 1:100-1:500 on frog and 1:100-1:2000 on chicken.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 50% glycerol. No preservative added.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to human Ductin (100% conserved in all species).
SpecificityRecognizes human ductin (the 16kD or c subunit of the V-ATPase). Has been specifically tested on flatworm (invertebrate), frog (vertebrate) and chick embryo tissue.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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