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Dulbecco’s MEM (DMEM) Ham’s F-12 w/o L-Glutamine, Phenol Red, Pyridoxal HCI (Powder)
Dulbecco’s MEM (DMEM) Ham’s F-12 w/o L-Glutamine, Phenol Red, Pyridoxal HCI (Powder)
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| Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM “High Glucose“ with 4500mg/ml aides in the cultivation of certain cell types. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells. | | | Catalog # | D9811-12 | | Ham's Nutrient Mixtures were originally developed to support growth of several clones of Chinese hamster ovary (CHO) cells, of HeLa and of mouse L-cells. Ham’s mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using CHO cells. | | Appearance | Off white, homogenous, free flowing powder | | Solubility | Amber, clear, complete | | pH | As reported | | Endotoxin | ~10EU/mg | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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