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You are here:Home » Culture Media » Media-Cell Culture, PBS » Dulbecco’s Phosphate Buffered Saline (PBS) (10X) w/CaCl2, MgCl2 (Powder)

Dulbecco’s Phosphate Buffered Saline (PBS) (10X) w/CaCl2, MgCl2 (Powder)

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Specifications

Dulbecco's Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromotographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process.
Catalog #D9821
Also AvailableD9819: Dulbecco’s Phosphate Buffered Saline (PBS) (Powder)
D9820: Dulbecco’s Phosphate Buffered Saline (PBS) w/o CaCl2, MgCl2 (Powder)
D9820-04: Dulbecco’s Phosphate Buffered Saline (PBS), Modified w/NaCl, NaH2PO4 w/o KCl, CaCl2, MgCl2 (Powder)
Dulbecco’s Phosphate Buffered Saline 10X (PBS) w/o CaCl2, MgCl2 (Powder)
D9821: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) (Powder)
D9822: Dulbecco’s Phosphate Buffered Saline (PBS) w/EDTA, w/o CaCl2, MgCl2 (Powder)
D9823: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) w/MgSO4 (Powder)
AppearanceWhite, homogeneous, free flowing powder
Solubility (1X)Colorless, clear and complete.
Some precipitate may be observed as a 10X due to excess concentration of salts. Sterile filter after solubilization.
pHAs Reported
Endotoxin1EU/ml
Formulation
Components g/L
Calcium Chloride•2H2O 1.325
Magnesium Chloride•6H2O 1.00
Potassium Chloride 2.00
Potassium Phosphate Mono 2.00
Sodium Chloride 80.00
Sodium Phosphate Dibasic 11.50
Total97.8g/L
Directions
To make a 10X solution
Dissolve 97.8g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers..
NoteSome precipitate may be observed due to excess concentration of salts. Sterile filter after solubilization.
USB recommends the preparation of this product as a 1X solution.
To make a 1X solution
Dissolve 9.78g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers.
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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