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You are here:Home » Antibodies » Abs to Enzymes, Cytochrome » Anti -Duodenal Cytochrome b (Dcytb)

Anti -Duodenal Cytochrome b (Dcytb)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
Iron is an essential element for a variety physiological process. A variety of proteins are involved in the transport of iron. Most dietary iron exists in the form of ferric iron complexes which must be reduced to yield ferrous ion before it can be taken up by protein transporters such as DCT1 (divalent cation transporter)/NRAMP2/DMT1. These transporters are especially active in small intestine where most dietary iron absorption is conducted. Ferrous Fe (II) is very unstable at physiological pH and quickly oxidized to ferric Fe (III). Therefore, highly specialized transmembrane electron transport system, maintained by ferric reductases, is required for the availability of intracellular ferrous ion.
Catalog #D9830-25
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 (ECL).
ELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Recommended Control PeptideD9830: Duodenal Cytochrome b, Mouse, Control Peptide (Dcytb)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PuritySerum
ImmunogenSynthetic peptide consisting of 20aa sequence located within the cytoplasmic C-terminus of mouse DCYTB (KLH). Species sequence homology
SpecificityRecognizes mouse Duodenal Cytochrome b (Dcytb). No significant sequene homology exists with b558 or other reductases.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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