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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B

Iron is an essential element for a variety physiological process. A variety of proteins are involved in the transport of iron. Most dietary iron exists in the form of ferric iron complexes which must be reduced to yield ferrous ion before it can be taken up by protein transporters such as DCT1 (divalent cation transporter)/NRAMP2/DMT1. These transporters are especially active in small intestine where most dietary iron absorption is conducted. Ferrous Fe (II) is very unstable at physiological pH and quickly oxidized to ferric Fe (III). Therefore, highly specialized transmembrane electron transport system, maintained by ferric reductases, is required for the availability of intracellular ferrous ion.
Catalog #	D9830-25
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000-1:5000 (ECL).
	ELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 1ug/ml.
	Optimal dilutions to be determined by the researcher.
Recommended Control Peptide	D9830: Duodenal Cytochrome b, Mouse, Control Peptide (Dcytb)
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	Not determined
Form	Supplied as a liquid in PBS, 0.05% sodium azide.
Purity	Serum
Immunogen	Synthetic peptide consisting of 20aa sequence located within the cytoplasmic C-terminus of mouse DCYTB (KLH). Species sequence homology
Specificity	Recognizes mouse Duodenal Cytochrome b (Dcytb). No significant sequene homology exists with b558 or other reductases.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.