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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Iron is an essential element for a variety physiological process. A variety of proteins are involved in the transport of iron. Most dietary iron exists in the form of ferric iron complexes which must be reduced to yield ferrous ion before it can be taken up by protein transporters such as DCT1 (divalent cation transporter)/NRAMP2/DMT1. These transporters are especially active in small intestine where most dietary iron absorption is conducted. Ferrous Fe (II) is very unstable at physiological pH and quickly oxidized to ferric Fe (III). Therefore, highly specialized transmembrane electron transport system, maintained by ferric reductases, is required for the availability of intracellular ferrous ion. A number of ferric reductases have been identified in yeast, plants, and bacteria but the identity of such proteins remained unknown in the mammals. Most recently, a new gene called Dcytb (for duodenal cytochrome b) has been cloned and characterized in mouse duodenum that may function as mammalian ferric reductase. Dcytb (human 286aa, rat 290aa) is a 6-transmembrane domains protein with ~45% sequence identity with cytochrome b561 reductase. No sequence homology exists with plant and yeast Dcytb. The N-terminus of mouse Dcytb is almost identical with the rabbit's p30 protein called cytochrome b558. Putative biding sites for cytochrome b561 substrates (ascorbic acid and semidehydroascorbic acid) are partially conserved in Dcytb and b561. Unlike most proteins involved in iron metabolism, Dcytb gene lacks a definable iron-responsive element. Dcytb is highly expressed in the brush border membrane of duodenal entrecotes.
Catalog #	D9830-30
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1-10ug/ml (ECL).
	ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
	Optimal dilutions to be determined by the researcher.
Control Peptide	D9830: Duodenal Cytochrome b, Mouse, Control Peptide (Dcytb)
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide, 40% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide consisting of 20aa sequence located within the cytoplasmic C-terminus of mouse DCYTB (KLH coupled).
Specificity	Recognizes mouse Duodenal Cytochrome b (Dcytb). No significant sequence homology exists with b558 or other reductases. Species Sequence Homology
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.