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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -DUSP1 (Dual Specificity Protein Phosphatase 1, CL100, Dual Specificity Protein Phosphatase hVH1, HVH1, Mitogen-activated Protein Kinase Phosphatase 1, MAP Kinase Phosphatase 1, MKP1, MKP-1, Protein-tyrosine Phosphatase CL100, PTPN10, VH1)

Anti -DUSP1 (Dual Specificity Protein Phosphatase 1, CL100, Dual Specificity Protein
Phosphatase hVH1, HVH1, Mitogen-activated Protein Kinase Phosphatase 1, MAP Kinase
Phosphatase 1, MKP1, MKP-1, Protein-tyrosine Phosphatase CL100, PTPN10, VH1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.
Catalog #D9840
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 2-4ug/ml, detects a band at 40kD which corresponds to the predicted MW of DUSP1.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide with sequence SYLQSPITTSPSC, aa355-367, from C-Terminus of human DUSP1.
SpecificityRecognizes human DUSP1. Species sequence homology: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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