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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » DUSP3, Recombinant, Human, aa1-185, His-Tag (Dual Specificity Protein Phosphatase 3, Dual Specificity Protein Phosphatase VHR, Vaccinia H1-related Phosphatase, VHR)

DUSP3, Recombinant, Human, aa1-185, His-Tag (Dual Specificity Protein Phosphatase 3, Dual
Specificity Protein Phosphatase VHR, Vaccinia H1-related Phosphatase, VHR)

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Specifications

DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DUSP3 is expressed in both breast and ovarian tissues. DUSP3 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.
Catalog #D9840-06M
SourceRecombinant corresponding to aa1-185 from human DUSP, fused to His-tag at N-terminal expressed in E.coli.
AA SequenceMGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP
Molecular Weight~22.6kD (MALDI-TOF)
Biological ActivityDetermined by measuring the amount of enzyme hydrolyzing 1nm of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH 7.5 using 10mM of substrate, corresponding to a specific activity of >1750IU/mg.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~22.6kD
SourceE.coli
Purity~95% (SDS-PAGE)
Concentration~1mg/ml
FormSupplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol, 2mM DTT, 0.15M sodium chloride.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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