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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH FC

Dynamin is a 100kD GTP binding protein that was first identified as a protein able to bind microtubules. A mutation in the Drosophila gene shits, encoding a dynamin homolog, prevents synaptic vesicle recycling and causes temperature-sensitive paralysis, implicating dynamin in the formation of coated vesicles. Dynamin may mediate vesicle formation by assembling into a ring structure around the necks of invaginated coated pits that may eventually lead to the closure of the neck and formation of vesicles. Amphiphysin, dynamin, clathrin and AP2 form a complex during synaptic vesicle endocytosis, which is regulated by the phosphorylated state of different components in this comple. To date, three genes have been found to encode dynamin isoforms. Dynamin 1 is neuronal specific. Dynamin 2 is ubiquitously expressed. Dynamin 3 localizes to testis, brain and lung.
Applications:
Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Catalog #	D9900-01
Recommended Dilutions	Western Blot (Colorimetric): 0.5ug/ml
	Flow Cytometry: 10ug/ml
	Immunohistochemistry: 1:50; Stains human colon cancer tissue. Staining of formaldehyde perfused and fixed rat brain shows synaptic immunofluorescence.
	Optimal dilutions to be determined by the researcher.
	Positive Control:
	Mouse Brain Tissue Extract
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	4E67
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant His 6-tagged human dynamin (aa1-750).
Epitope	GNRGMEDLIPLVNRLQD.
Specificity	Recognizes a 100kD protein, corresponding to human dynamin.
Species Crossreactivity	Mouse, rat, chicken and bovine. Sequence Homology: Dynamin and Dynamin 1 at 17/17 residues (aa2-18) and dynamin 2 and 3 at 15/17 residues.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.