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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Dynamin

Anti -Dynamin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC
Dynamin is a 100kD GTP binding protein that was first identified as a protein able to bind microtubules. A mutation in the Drosophila gene shits, encoding a dynamin homolog, prevents synaptic vesicle recycling and causes temperature-sensitive paralysis, implicating dynamin in the formation of coated vesicles. Dynamin may mediate vesicle formation by assembling into a ring structure around the necks of invaginated coated pits that may eventually lead to the closure of the neck and formation of vesicles. Amphiphysin, dynamin, clathrin and AP2 form a complex during synaptic vesicle endocytosis, which is regulated by the phosphorylated state of different components in this comple. To date, three genes have been found to encode dynamin isoforms. Dynamin 1 is neuronal specific. Dynamin 2 is ubiquitously expressed. Dynamin 3 localizes to testis, brain and lung.
Applications:
Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Catalog #D9900-01
Recommended DilutionsWestern Blot (Colorimetric): 0.5ug/ml
Flow Cytometry: 10ug/ml
Immunohistochemistry: 1:50; Stains human colon cancer tissue. Staining of formaldehyde perfused and fixed rat brain shows synaptic immunofluorescence.
Optimal dilutions to be determined by the researcher.
Positive Control:
Mouse Brain Tissue Extract
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4E67
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant His 6-tagged human dynamin (aa1-750).
EpitopeGNRGMEDLIPLVNRLQD.
SpecificityRecognizes a 100kD protein, corresponding to human dynamin.
Species CrossreactivityMouse, rat, chicken and bovine. Sequence Homology: Dynamin and Dynamin 1 at 17/17 residues (aa2-18) and dynamin 2 and 3 at 15/17 residues.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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