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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Dynamin, phosphorylated (Ser774)

Anti -Dynamin, phosphorylated (Ser774)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B IH IC IF
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.
Catalog #D9901-20
ApplicationsSuitable for use in Immunofluorescence, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1:1000
Western Blot: 1:1000; Band at ~95kD. The labeling of the dynamin band in Western Blots of rat brain was blocked by the phosphopeptide used as the immunogen, but not by the corresponding dephosphopeptide.
Immunocytochemistry: 1:1000
Immunohistochemistry: 1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlRat brain synaptosomal lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser774 of human, mouse and rat dynamin.
SpecificityRecognizes human dynamin. Labels dynamin phosphorylated in vitro by Cdk5, but not PKC. Does not crossreact with other purified substrates of Cdk5 (eg. amphiphysin and synapsin).
Species CrossreactivityMouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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