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You are here:Home » Antibodies » Abs to Apoptosis » Anti -DYNLT3 (Dynein light chain Tctex-type 3, RCG42244, isoform CRA_a, Tcte1l)

Anti -DYNLT3 (Dynein light chain Tctex-type 3, RCG42244, isoform CRA_a, Tcte1l)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Eukaryotic cells rely on actin and microtubule-based protein "motors" to generate intracellular movements. These motor proteins contain specialized domains that hydrolyse ATP to produce force and movement along a cytoskeletal polymer. In all cell types, dynein is a large protein complex composed of six distinct subunits and is responsible for most intracellular movement toward the minus ends of microtubules. Cytoplasmic dynein has many important roles in transporting membrane bound organelles, including endosomes, lysosomes, and mitochondria. Cytoplasmic dynein is also responsible for virus transport to the nucleus, retrograde axonal transport, and microtubule and neurofilament transport. Three families of dynein light chains (DYNLT) have been identified. Two isoforms of the DYNLT family of light chains, DYNLT1 and DYNLT3, have been proposed to link dynein to specific cargoes. Recent publications indicate that DYNLT3 binds to Bub3, a spindle checkpoint protein and Bub3 binds exclusively to DYNLT3 and not to other dynein light chains. DYNLT3 is present on kinetochores at prometaphase, but not later mitotic stages.It has been suggested that a subpopulation of dynein, containing the DYNLT3 light chain, may have a role in the transport of checkpoint proteins from the kinetochore to the spindle pole.
Catalog #D9901-65A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5ug/ml
Immunohistochemistry (Paraffin): 1:50
Optimal dilutions to be determined by the researcher.
Positive ControlRat brain lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No9H226
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenFull-length recombinant rat DYNLT3 protein.
SpecificityRecognizes rat DYNLT3. Species Crossreactivity: human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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