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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B

Catalog #	D9905-05
Applications	Suitable for use in ELISA, Inhibition of Laminin Binding to Dystroglycan and Western Blot. Other applications not tested.
Recommended Dilutions	Inhibition of Laminin Binding to Dystroglycan: In a nitrocellulose overlay experiment, this antibody inhibits binding of 125I Laminin to Dystroglycan.
	Western Blot: 1:1000-1:2000 Detects alpha-Dystroglycan in rabbit skeletal muscle. Rabbit skeletal muscle lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with D9905-05. Proteins were visualized using an IgM (HRP) goat anti-mouse and a chemiluminescence detection system.
	Note: The use of WGA puri ed protein results in signi cantly cleaner blots and immunoprecipitates. Post-translational modi cation of Dystroglycan causes band broadening.
	Optimal dilutions to be determined by the researcher.
Recommended Positive Control	Rabbit skeletal muscle lysate
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	4A145
Host	Mouse
Source	Rabbit
Concentration	Not determined
Form	Supplied as a liquid in PBS, 0.05% sodium azide, 30% glycerol.
Purity	Ascites
Immunogen	Rabbit skeletal muscle membrane preparation.
Specificity	Recognizes rabbit alpha-Dystroglycan at ~156kD. Species Crossreactivity: human, mouse, rat, canine and guinea pig.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.