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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Dystroglycan, beta (Beta-Dystroglycan, Beta-DG)

Anti -Dystroglycan, beta (Beta-Dystroglycan, Beta-DG)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH
Beta-dystroglycan is found in normal muscle but has a differential expression in certain instances of muscular dystrophy. This antibody will help identify the presence or absence of beta-dystroglycan.
Catalog #D9905-07A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Not suitable for paraffin sections. Other applications not tested.
Recommended DilutionsWestern Blot: 1:25-1:50
Immunohistochemistry (Frozen): 1:50-1:200, unfixed sections for 1 hour at RT
Optimal dilutions to be determined by the researcher.
Positive Control
Western Blot: Skeletal muscle, band at ~43kD.
Immunohistochemistry: Striated muscle, Continuous staining at periphery of muscle fibers.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No43DAG1/8D5
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyophilized powder, 15mM sodium azide. Reconstitute with 1ml sterile ddH2O.
PuritySupernatant
ImmunogenSynthetic peptide containing 15 of the last 16aa at the end of the C-terminal region of the human dystroglycan, PKNMTPYRSPPPYVP-PCOOH.
SpecificityRecognizes human Dystrogycan. Species Crossreactivity: canine, chicken, hamster, mouse, porcine, rabbit and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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