| Dystrophin is a member of the spectrin/alpha-actinin family of actin-binding, triple helix rod-containing proteins. It is absent or greatly reduced in individuals with the X-linked recessive Duchenne’s muscular dystrophy disorder, as well as in mice with the mdx (murine muscular dystrophy) mutation. |
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| Catalog # | D9910-30 |
| Applications | Suitable for use in Western Blot, Immunohistochemistry and EM Gold. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:100-1:250 |
| Immunohistochemistry(Fresh frozen, unfixed tissue): Neat 1:20 |
| Not recommended for use on paraffin embedded tissue. |
| EM Gold: Undiluted 90 minute incubation at 25°C. Light fixation with 2% formaldehyde + 0.001% glutaraldehyde for 1 hour. Use 2.3M sucrose as cryoprotectant. |
| Optimal dilutions to be determined by the researcher. |
| Expected Staining Pattern | Light microscopy: Continuous rim of labeling at the periphery of muscle fibers |
| Western Blot: Strong double bands at ~400kD and metabolites of lower molecular mass |
| E.M. Gold: Close to the cytoplasmic face of the plasma membrane. |
| Positive Control | Snap frozen normal human or rat striated muscle. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 2Q1101 |
| Host | Mouse |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid in PBS, 1% BSA, 15mM sodium azide. |
| Purity | Supernatant |
| Immunogen | Bacterial fusion protein |
| Specificity | Recognizes mid rod domain of human dystrophin between aa1181-1388. Reacts with skeletal, cardiac and smooth muscle dystrophin. Reacts on blots with the brain isoform. No reactivity with mdx mouse tissue or DMD/BMD patients who have a gene deletion. Does not react with chicken dystrophin. Species Crossreactivity: mouse, rat, rabbit and canine |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
