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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Dystrophin, Rod Domain

Anti -Dystrophin, Rod Domain

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH
Dystrophin is a member of the spectrin/alpha-actinin family of actin-binding, triple helix rod-containing proteins. It is absent or greatly reduced in individuals with the X-linked recessive Duchenne’s muscular dystrophy disorder, as well as in mice with the mdx (murine muscular dystrophy) mutation.
Catalog #D9910-30
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and EM Gold. Other applications not tested.
Recommended DilutionWestern Blot: 1:100-1:250
Immunohistochemistry(Fresh frozen, unfixed tissue): Neat 1:20
Not recommended for use on paraffin embedded tissue.
EM Gold: Undiluted 90 minute incubation at 25°C. Light fixation with 2% formaldehyde + 0.001% glutaraldehyde for 1 hour. Use 2.3M sucrose as cryoprotectant.
Optimal dilutions to be determined by the researcher.
Expected Staining PatternLight microscopy: Continuous rim of labeling at the periphery of muscle fibers
Western Blot: Strong double bands at ~400kD and metabolites of lower molecular mass
E.M. Gold: Close to the cytoplasmic face of the plasma membrane.
Positive ControlSnap frozen normal human or rat striated muscle.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No2Q1101
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 1% BSA, 15mM sodium azide.
PuritySupernatant
ImmunogenBacterial fusion protein
SpecificityRecognizes mid rod domain of human dystrophin between aa1181-1388. Reacts with skeletal, cardiac and smooth muscle dystrophin. Reacts on blots with the brain isoform. No reactivity with mdx mouse tissue or DMD/BMD patients who have a gene deletion. Does not react with chicken dystrophin. Species Crossreactivity: mouse, rat, rabbit and canine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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