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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -Dystrophin (BSA & Azide Free)

Anti -Dystrophin (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH IF
Dystrophin is a member of the spectrin/alpha-actinin family of actin binding, triple helix rod containing proteins. It is absent or greatly reduced in individuals withe the X-linked recessive Duchenne’s muscular dystrophy disorder, as well as in mice with the mdx, (murine muscular dystrophy) mutation.
Catalog #D9910X
ApplicationsSuitable for use in Affinity Purification, Electron Microscopy, ELISA, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Formalin/Paraffin): 2-4ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Note that 1mM EDTA, pH 8.0 is better than 10mM citrate buffer, pH 6.0 for unmasking the epitope.
Optimal dilutions to be determined by the researcher.
Positive ControlSkeletal muscle
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No0.N.258
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See D9910.
PurityPurified by Protein G affinity chromatography.
ImmunogenAcetylcholine receptor (AChR)-enriched membranes and peripheral membrane proteins from Torpedo nobiliana electric organ.
Cellular LocalizationCell membrane.
SpecificityRecognizes human Dystrophin at ~427kD. Does not react with C protein, a-actin, or human muscle spectrin. Species Crossreactivity: mouse, rat, chicken, Xenopus, Torpedo nobiliana
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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