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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH IF

Dystrophin is a member of the spectrin/alpha-actinin family of actin binding, triple helix rod containing proteins. It is absent or greatly reduced in individuals withe the X-linked recessive Duchenne’s muscular dystrophy disorder, as well as in mice with the mdx, (murine muscular dystrophy) mutation.
Catalog #	D9910X
Applications	Suitable for use in Affinity Purification, Electron Microscopy, ELISA, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (Formalin/Paraffin): 2-4ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Note that 1mM EDTA, pH 8.0 is better than 10mM citrate buffer, pH 6.0 for unmasking the epitope.
	Optimal dilutions to be determined by the researcher.
Positive Control	Skeletal muscle
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.258
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See D9910.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Acetylcholine receptor (AChR)-enriched membranes and peripheral membrane proteins from Torpedo nobiliana electric organ.
Cellular Localization	Cell membrane.
Specificity	Recognizes human Dystrophin at ~427kD. Does not react with C protein, a-actin, or human muscle spectrin. Species Crossreactivity: mouse, rat, chicken, Xenopus, Torpedo nobiliana
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.