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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

The recombinant protein over-expression systems with well-known epitope tags are powerful strategies in checking and purifying the interest proteins, which are expressed in certain vectors containing the particular tagged fragments. The small tags are used to be cloned along with the target proteins, and the antibodies against those fusion-tags are used to monitor fusion protein expression and purification. E-Tag (GAPVPYPDPLEPR) is a popular epitope tag incorporated in the amino or carboxy terminus of the target protein. This antibody can be used to check E- tagged fusion proteins.
Catalog #	E0004-01K
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 0.5-2ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to the GAPVPYPDPLEPR sequence.
Specificity	Recognizes E-Tag in all species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.