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You are here:Home » Antibodies » Abs to Retinoblastoma Proteins » Anti -E2F1 Transcription Factor, phosphorylated (Ser364) (E2F-1, OTTHUMP00000030661, PBR3, PRB-binding Protein E2F-1, Retinoblastoma Associated Protein 1, RBAP1, RBAP-1, Retinoblastoma Binding Protein 3, RBBP3, RBP3)

Anti -E2F1 Transcription Factor, phosphorylated (Ser364) (E2F-1, OTTHUMP00000030661, PBR3,
PRB-binding Protein E2F-1, Retinoblastoma Associated Protein 1, RBAP1, RBAP-1,
Retinoblastoma Binding Protein 3, RBBP3, RBP3)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
E2F1 can also mediate p53-dependent and independent apoptosis, and phosphorylation of E2F1 at serine 364 results in its stabilisation and apoptosis induction.
Catalog #E0010-01F
E2F1 is a target of the transforming proteins of small DNA tumor viruses and increased expression of this protein has been reported in a variety of cancers.
ApplicationsSuitable for use in ELISA, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (paraffin): 2-20ug/ml
ELISA: 1:10,000-1:60,000
Western Blot: 1:150-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to aa360-369 of human E2F1.
SpecificityRecognizes human E2F1 when phosphorylated at Ser364. Species sequence homology: chimpanzee.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesKIAA4009


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