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You are here:Home » Antibodies » Abs to Retinoblastoma Proteins » Anti -E2F1 Transcription Factor (E2F 1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3)

Anti -E2F1 Transcription Factor (E2F 1, Retinoblastoma associated protein 1,
Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IF
Clone 16G7 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.
Catalog #E0010-02F
Positive Control E2F-1 transfected Soas2 cells and recombinant fusion protein.
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No8k17
HostMouse
SourceHuman
ConcentrationAs reported
FormAs reported
PurityMouse ascites
ImmunogenFusion protein containing the region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.
SpecificityThis antibody is specific for human E2F-1. Species Crossreactivity: human E2F-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesE2F 1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP-3, RBBP3, RBP3


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