| 5'-G A C N N N^N N G T C-3' |
|
| 3'-C T G N N^N N N C A G-5' |
|
| Catalog # | E0200-05 |
| Concentration | 10u/ul |
| Source: Enterobacter amnigenus RFL1105 |
| Supplied with | R1625-50: Restriction Enzyme Buffer for E0200-05, 10X |
| 10mM Tris-HCl (pH 7.5), 5mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA. Incubate at 37°C |
| Diluent Buffer | 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. |
| Storage Buffer | 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. |
| Overdigestion Assay | No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours). |
|
| Ligation/Recutting Assay | After 16-fold overdigestion (1u/ug DNA x 16 hours) with Eam1105I, more than 80% of the pUC19 DNA fragments can be ligated in a reacton mixture containing 20–40u of T4 DNA ligase/1ug of fragments and 10% PEG at a 5'-termini concentration of 1.8uM. More than 90% of these can be recut. |
|
| Labeled Oligonucleotide (LO) Assay | No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. |
|
| Star Activity | An excess of enzyme (7.5u/ug DNA x 16 hours) may result in star activity. |
|
| Stability during Prolonged Incubation | A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. |
|
| Thermal Inactivation | Enzyme is inactivated by incubation at 65°C for 20min. |
|
| Digestion of Agarose-embedded DNA | A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. |
