Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » MB-Enzymes, Restriction » Eam1105I



  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.


5'-G A C N N N^N N G T C-3'
3'-C T G N N^N N N C A G-5'
Catalog #E0200-05
Concentration 10u/ul
Source Enterobacter amnigenus RFL1105
Supplied withR1625-50: Restriction Enzyme Buffer, 10X for E0200-05
Supplied in a 10X concentrated stock. Dilute to 1X or 2X before use.
When used as a 1X liquid the form is 10mM Tris-HCl, 5mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA. Incubate at 37°C
R1625 Restriction Enzyme Buffer A, 10X
Supplied in a 10X concentrated stock. Dilute to 1X or 2X before use.
When used as a 1X liquid the form is 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA
Storage BufferSupplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours).
Ligation/Recutting Assay
The ligation and recutting assay was replaced with LO test ater validating experiments showed LO test ability to trace nuclease and phosphate activities with sensitivity that is higher than L/R by a factor of 10.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of E0200-05 for 4 hours.
Star Activity
An excess of enzyme (7.5u/ug DNA x 16 hours) may result in star activity.
Stability during Prolonged IncubationA minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal InactivationEnzyme is inactivated by incubation at 65°C for 20min.
Digestion of Agarose-embedded DNAA minimum of 5 units of Eam11051 is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Number of Recognition Sites in DNA
PhiX174, pTZ19R/U, pBR322, pUC18/19, pUC571
Enzyme Properties
Methylation Effects on Digestion
Dam, Dcm, EcoKl, EcoBlNever overlaps - no effect
CpGMay overlap - no effect

External Links