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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » Eam1105I



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5'-G A C N N N^N N G T C-3'
3'-C T G N N^N N N C A G-5'
Catalog #E0200-05
Concentration 10u/ul
Source: Enterobacter amnigenus RFL1105
Supplied withR1625-50: Restriction Enzyme Buffer for E0200-05, 10X
10mM Tris-HCl (pH 7.5), 5mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA. Incubate at 37°C
Diluent Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours).
Ligation/Recutting AssayAfter 16-fold overdigestion (1u/ug DNA x 16 hours) with Eam1105I, more than 80% of the pUC19 DNA fragments can be ligated in a reacton mixture containing 2040u of T4 DNA ligase/1ug of fragments and 10% PEG at a 5'-termini concentration of 1.8uM. More than 90% of these can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Star ActivityAn excess of enzyme (7.5u/ug DNA x 16 hours) may result in star activity.
Stability during Prolonged IncubationA minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal InactivationEnzyme is inactivated by incubation at 65°C for 20min.
Digestion of Agarose-embedded DNAA minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.

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