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You are here:Home » Antibodies » Abs to Glucose Transporters » Anti -EAT-4 (Vesicular Type Transporters for Glutamate, VGLUT)

Anti -EAT-4 (Vesicular Type Transporters for Glutamate, VGLUT)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #E0255-05
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique. EAT-4 is ~65kD protein.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
PurityAffinity purified over the control-peptide Sepharose
ImmunogenAn 18 AA synthetic peptide witin the N-terminus of C. elegans EAT-4 was synthesized, conjugated to KLH
SpecificityThe peptide has no significant sequence with mammalian VGLUT1/2 or other NaPi-related (Type-1 to Type-III) transporters. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10 ug per 1 ul of antiserum or 1 ug of aff pure IgG) to confirm antibody specificity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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