EBAG9, Recombinant, Human (Estrogen Receptor-binding Fragment-associated Gene 9 Protein, EB9, Cancer-associated Surface Antigen RCAS1, PDAF, Receptor-binding Cancer Antigen Expressed on SiSo Cells, RCAS1)
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| Estrogen receptor binding site associated antigen 9, also known as EBAG9, is a type III transmembrane protein that is predominantly located in the Golgi. This protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers, such as advanced breast and prostate cancers. As the EBAG9 acts to inhibit the growth of receptor-binding cells and induced apoptosis of immune cells, cancer cells might evade immune surveillance. So immunodetection of EBAG9 expression can be a negative prognostic indicator. | | | Catalog # | E0255-55B | | Recombinant human EBAG9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. | | Source | Recombinant corresponding to aa28-213 of human EBAG9 (His-tagged) expressed in E. coli. | | AA Sequence | MGSSHHHHHH SSGLVPRGSH MRSGRGRKLS GDQITLPTTV DYSSVPKQTD VEEWTSWDED APTSVKIEGG NGNVATQQNS LEQLEPDYFK DMTPTIRKTQ KIVIKKREPL NFGIPDGSTG FSSRLAATQD LPFIHQSSEL GDLDTWQENT NAWEEEEDAA WQAEEVLRQQ KLADREKRAA EQQRKKMEKE AQRLMKKEQN KIGVKLS | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 3ug/ml 15% SDS-PAGE, ~23.4kD | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. | | Source | E. coli | | Purity | Purified 90% by SDS PAGE. | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 20mM Tris pH8.0,1mM DTT, 2mM EDTA, 10% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Estrogen Receptor Binding Site Associated, Antigen, 9, HGNC:3123 |
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