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You are here:Home » Antibodies » Abs to Binding Proteins » Anti -EBP50 (ERM (Ezrin-Radixin-Moesin) Binding Phosphoprotein of 50kD, Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1, NHERF, NHERF1, NHERF-1, NPHLOP2, Regulatory Cofactor of Na(+)/H(+) Exchanger, Sodium Hydrogen Exchanger Regulatory Factor 1, Solute Carrier

Anti -EBP50 (ERM (Ezrin-Radixin-Moesin) Binding Phosphoprotein of 50kD, Na(+)/H(+)
Exchange Regulatory Cofactor NHE-RF1, NHERF, NHERF1, NHERF-1, NPHLOP2, Regulatory Cofactor
of Na(+)/H(+) Exchanger, Sodium Hydrogen Exchanger Regulatory Factor 1, Solute Carrier

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50kD] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.
Catalog #E1050
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1ug/ml
Immunohistochemistry: Staining of EBP50 in mouse airway results in staining of the epithelium.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide. Also available with Biotin and without BSA or azide. See E1050B.
PurityPurified by affinity chromatography.
ImmunogenRecombinant human His-tagged EBP 50 obtained from bacteria. All His-tag specific antibodies have been removed.
SpecificityRecognizes EBP 50 from human T84 and Calu3 cells as well as recombinant human protein at ~50kD. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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