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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Enterokinase is an intestinal enzyme responsible for initiating activation of pancreatic proteolytic proenzymes. Mouse Anti-Enterokinase Monoclonal Antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant EK protein (bovine EK, aa848-1035, light chain, 93% homologous with human EK).
Catalog #	E2000-01B
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 0.05-0.2ug/ml
	Western Blot: 0.1-1.0ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	9E4
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder in PBS, PBS, pH 7.4, 0.02% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Immunized with purified recombinant EK protein (bovine EK, aa848-1035, light chain, 93% homologous with human EK).
Specificity	Recognizes EK in Western blots and ELISAs
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.