| Catalog # | E2000-07A |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | ELISA: 1:25,000 to yield OD=1 in a 15 minute reaction with TMB as a substrate. |
| Western Blot: 0.2-1.0ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Western Blot Control | P9106-52: Protein MW Marker, 12 Tag |
| E. coli lysate containing a 41kD protein expressing 12 commonly used tags which are fused in the order of GST, T7, HSV, c-MYC, VSV, Glu-Glu, alpha Tubulin, V5, E-tag, FLAG, S-tag, 6xHIS. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS pH 7.2, 0.1% sodium azide before the addition of glycerol to 40%. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | FLAG peptide corresponding to aa sequence DYKDDDDK (KLH coupled). |
| Specificity | Recognizes FLAG epitopes. Antibody was anayzed using amino-terminal Met-Flag-BAP, amino-terminal FLAG-BAP and carboxy-terminal FLAG-BAP fusion proteins. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
