| Catalog # | E2000-07B |
| Applications | Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000-1:5000 |
| ELISA: 1:5000-1:10,000 |
| Immunofluorescence: 1:250-1:1000 |
| Optimal dilutions to be determined by the researcher. |
| Western Blot Control | Protein MW Marker, 12 Tag, Catalog No: P9106-52 is an E. coli lysate containing a 41kD protein expressing commonly used tags which are fused in the order of GST , T7, HSV, cMYC , VSV, Glu-Glu, V5, E-tag, FLAG, S-tag, HA, 6xHIS. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS pH 7.0, 0.2% BSA, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC). Label Ratio |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | FLAG peptide corresponding to aa sequence DYKDDDDK (KLH coupled). |
| Specificity | Recognizes FLAG epitopes. Antibody was anayzed using amino-terminal Met-Flag-BAP, amino-terminal FLAG-BAP and carboxy-terminal FLAG-BAP fusion proteins. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
