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You are here:Home » Antibodies » Abs to Cell Markers » Anti -ECT2 (Protein ECT2, Epithelial Cell-transforming Sequence 2 Oncogene)

Anti -ECT2 (Protein ECT2, Epithelial Cell-transforming Sequence 2 Oncogene)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.
Catalog #E2050-01A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 2.5ug/ml, in human U87-MG glioblastoma lysate on ~100kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to AENSVLTSTTGRT-C, from human ECT2, at N-terminal (NP_060568.3).
SpecificityRecognizes human ECT2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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