Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -EDD (E3 Ubiquitin-protein Ligase UBR5, E3 Ubiquitin-protein Ligase, HECT Domain-containing 1, Hyperplastic Discs Protein Homolog, hHYD, Progestin-induced Protein, UBR5, EDD1, HYD, KIAA0896)

Anti -EDD (E3 Ubiquitin-protein Ligase UBR5, E3 Ubiquitin-protein Ligase, HECT
Domain-containing 1, Hyperplastic Discs Protein Homolog, hHYD, Progestin-induced Protein,
UBR5, EDD1, HYD, KIAA0896)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
UBR5 (Ubiquitin protein ligase E3 component n-recognin 5; also HYD and EDD) is a 280-30kD member of the HECT E3 family of ubiquitin (Ub)protein ligases. It is ubiquitously expressed, and found in both the cytoplasm and nucleus of cells. In addition to ubiquination, UBR5 is functionally diverse. In the cytoplasm, it interacts with, and stabilizes APC, thereby increasing its concentration. In the nucleus (of smooth muscle cells), it stabilizes myocardin, a transcription factor that activates smooth muscle-specific genes. Perhaps its best known function involves DNA checkpoint activation. Following DNA damage, UBR5 promotes the phosphorylation of CHK2, which arrests the cell cycle at G2/M. Human UBR5 is 2799aa in length and contains a Ub-associated domain (aa180-215), two NLS’s (aa502-517 and 630-635), one UBR-type zinc finger region (aa1177-1244), a PABC region (aa2377-2454) and a HECT domain with a Ub-binding Cys residue (aa2462-2799). Over (aa1-227), human UBR5 shares 98% aa identity with mouse UBR5.
Catalog #E2202-50E
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5ug /ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa1-227 from UBR5 expressed in E. coli (095071).
SpecificityRecognizes human, mouse and rat UBR5.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links