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You are here:Home » Antibodies » Abs to G Protein Receptors » Anti -EDG1 (Endothelial Cell Differentiation Gene 1)

Anti -EDG1 (Endothelial Cell Differentiation Gene 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
EDG1 is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. EDG1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis and angiogenesis.
Catalog #E2208-01C
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume 200ul.
Optimal dilutions to be determined by the researcher.
HybridomaMyeloma cells with spleen cells from Balb/c mice with NS0-derived, recombinant human Endothelial Differentiation Gene 1 (hEDG1).
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No8H120
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0 cells transfected with human EDG1.
SpecificityRecognizes human EDG1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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