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Anti -EDG1 (Endothelial Cell Differentiation Gene 1) (PE)
Anti -EDG1 (Endothelial Cell Differentiation Gene 1) (PE)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
FC |
|
| EDG1 is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. EDG-1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis and angiogenesis. | | | Catalog # | E2208-01E | | Applications | Suitable for use in Flow Cytometry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: Neat. 10ul labels 10e6 cells. Validated using human EDG1 transfected CHO-S cells. Cell surface expression is determined using 488nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 565-605nm. | | Optimal dilutions to be determined by the researcher. | | Flow Cytometry Protocol | 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. | | 2. Add 10ul of E2208-01E to 1x10e6 cells and incubate for 30 minutes at RT. | | 3. Removed unbound antibody by washing the cells twice with Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point. | | 4. Resuspend the cells in F4568-10 for analysis. As a control, cells in a separate tube should be treated with PE-labeled mouse IgG2b antibody. | | Storage and Stability | May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 8H120 | | Host | Mouse | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE). | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | NS0 cells transfected with human EDG1 (aa1-382; Accession#P21453) | | Specificity | Recognizes human EDG1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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