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You are here:Home » Antibodies » Abs to G Protein Receptors » Anti -EDG1 (Endothelial Cell Differentiation Gene 1) (PE)

Anti -EDG1 (Endothelial Cell Differentiation Gene 1) (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
EDG1 is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. EDG-1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis and angiogenesis.
Catalog #E2208-01E
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Neat. 10ul labels 10e6 cells. Validated using human EDG1 transfected CHO-S cells.
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. Add 10ul of E2208-01E to 1x10e6 cells and incubate for 30 minutes at RT.
3. Removed unbound antibody by washing the cells twice with Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point.
4. Resuspend the cells in F4568-10 for analysis. As a control, cells in a separate tube should be treated with PE-labeled mouse IgG2b antibody.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No8H120
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa1-382 from human EDG1, expressed in NS0 cells (P21453).
SpecificityRecognizes human EDG1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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