Anti -EDG 2 (Endothelial Cell Differentiation Gene 2, LPA1, Lysophosphatidic acid 1,
Ventricular Zone Gene-1, vzg-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IC |
|
| Endothelial Cell Differentiation Gene-2, or EDG-2, encodes a 50kD protein belonging to the family of G-Protein Coupled Receptors (GPCR’s), and shows a strong affinity for LPA. To date, 8 members of the EDG receptor family have been identified. These receptors are suggested to have a role in modulation of growth-related effects including cell proliferation and differentiation as well as protective, anti-apoptotic effects. EDG receptor expression is developmentally regulated and the expression of the 8 members of this family of proteins appears to be tissue specific. EDG receptors couple to G-proteins to signal through Ras and MAPK pathways and other protein tyrosine kinase pathways. EDG-2 is also known as LPA1. The ligand for EDG-2 is lysophosphatidic acid (LPA). EDG-2 is expressed by oligodendrocytes and some human malignant T-cell lines. EDG-2 was originally identified in the ventricular zone of the developing cerebral cortex. Together with EDG-4, EDG-2 may have anti-apoptotic effects in cardiomyocytes subjected to hypoxia and adrenergic stimulation. | | | LPA1 (also known as EDG-2) is one of four known LPA receptors (LPA1-4).1 LPA1-3 are part a family of G protein-coupled receptors that share identity to the sphingosine-1-phosphate receptors (S1P1-5). LPA1 couples with three types of G proteins, Gi/o, Gq, and G12/13 to induce a range of cellular responses including activation of phospholipase C, multiple kinases, the serum response element and cell proliferation. LPA1 mRNA is detected in a wide range of tissues and cells including brain, heart, small intestine, spleen, testis, kidney, HeLa cells, and A549 cells. Murine and human LPA1 have 364 amino acids with an estimated molecular weight of 41kD. | | | Catalog # | E2208-10B | | Applications | Suitable for use in Western Blot and Immunocytochemistry. | | Other applications not tested. | | Recommended Dilution | Western Blot: 5ug/mL | | Immunocytochemistry: 8ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in TBS, pH 7.4, 0.1% BSA, 0.02% sodium azide, and 50% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Human lysophosphatidic acid 1 (LPA1) amino acids 342-359 (DRSASSLNHTILAGVHSN) | | Specificity | Recognizes EDG-2 (50kD). Detects the protein from several species from 43-50kD, suggesting heterogeneous LPA1 post-translational modifications in distinct cells. | | Species crossreactivity | Human, bovine, murine, rat, and ovine. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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