Anti -EDG 2 (Endothelial Cell Differentiation Gene 2, LPA1, Lysophosphatidic acid 1,
Ventricular Zone Gene-1, vzg-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| The endothelial differentiation gene (Edg) family, a subfamily of G protein coupled receptors, is a specific mediator of lysophospholipids. Edg-2, also known as ventricular zone gene-1 (vzg-1), mediates many of the biological activities of lysophoshatidic acid (LPA). Heterologous expression of Edg-2 in neuronal and non-neuronal cell types produces diverse cellular responses which include increases in [ 3H]LPA-binding sites, G protein activation, stress fiber formation, neutrite retraction, serum response element (SRE) activation, increased DNA synthesis, and suppression of apoptosis. While Edg-2 mediates many of the biological activities of LPA, the contribution of non-receptor mechanisms to LPA signaling remains unclear. | | | Catalog # | E2208-11A | | Applications | Suitable for use in Western Blot. Other applications have not been tested. | | Recommended Dilutions | Western Blot: 0.5-2ug/ml detects Edg-2 in rat brain microsomal preparation. The specificity of the antibody in Western Blot was confirmed by peptide inhibition using the immunizing peptide. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with E2208-1A (2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Optimal dilutions to be determined by the researcher. | | Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb | | I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb | | I1904-46Q: IgG, H&L (HRP) Pab Gt xRb | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of 30% glycerol. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa328-344, CQRSENPTGPTESSDRS of human Edg-2, conjugated to KLH | | Specificity | Recognizes human Edg-2 at ~50kD. Species Crossreactivity: rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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