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Anti -EDG 5 (Endothelial Cell Differentiation Gene 5)
Anti -EDG 5 (Endothelial Cell Differentiation Gene 5)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| Endothelial Cell Differentiation-5, or EDG-5, encodes a 45kD protein belonging to a family of G-Protein Coupled Receptors (GPCR’s), which bind lysophospholipids. To date, 8 members of the EDG receptor family have been identified. These receptors are suggested to have a role in modulation of growth-related effects including cell proliferation and differentiation as well as protective, anti-apoptotic effects. EDG receptor expression is developmentally regulated and the expression of the 8 members of this family of proteins appears to be tissue specific. EDG receptors couple to G-proteins to signal through Ras and MAPK pathways and other protein tyrosine kinase pathways. EDG-5 is also known as S1P2. The ligand for EDG-5 is sphingosine-1-phosphate. Ligand binding inhibits Rac and growth factor induced chemotaxis. EDG-5 is 45-60% homologous with EDG-1, EDG-3 and EDG-8, indicated by amino acid sequence analysis. EDG-5 is expressed by human epithelial cells and fibroblasts, as well as T-cells, ovarian tumor, breast carcinoma, epithelial, spleen, lung cells and some lymphoblastoma cell lines. | | | Catalog # | E2208-25 | | Hybridoma | BALB/c mouse derived hybridoma | | Applications | Suitable for use in Western Blotting. Other applications not tested. | | Recommended Dilution | Western Blotting: 1-10ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 2B2.13 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid (purified immunoglobulin) in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide | | Purity | Purified from tissue culture supernatant by Protein A affinity chromatography. Sterile filtered. | | Immunogen | Synthetic peptide corresponding to the C terminal region of the human EDG-5 protein. | | Specificity | Recognizes EDG-5 and does not crossreact with EDG-2, EDG-3 or EDG-4. Species Reactivity: Human and rat. | | | Important Note | This product is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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