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You are here:Home » Antibodies » Abs to Cell Markers » Anti -EEA1 (Early Endosomal Antigen 1, Early Endosome Associated Protein, Endosome Associated Protein p162, ZFYVE2, Zinc Finger FYVE Domain Containing Protein 2)

Anti -EEA1 (Early Endosomal Antigen 1, Early Endosome Associated Protein, Endosome
Associated Protein p162, ZFYVE2, Zinc Finger FYVE Domain Containing Protein 2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B Gel
Early endosomal antigen 1 (EEA1) is a 162kD membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.
Catalog #E2211-01E
ApplicationsSuitable for use in Dot Blot and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8D102
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 7.4, 1% BSA and 0.05% sodium azide.
PurityPurified using Protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum.
ImmunogenPartial sequence of recombinant full-length protein to human EEA1
SpecificityRecognizes human EEA1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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