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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -Early Endosome Antigen 1 (EEA1)

Anti -Early Endosome Antigen 1 (EEA1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IC
Early endosome antigen 1 (EEA1) is a peripheral membrane protein that contains a calmodulin-binding IQ motif and cysteine rich finger motif necessary for its specific localization to the early endosomes. These early endosomes are cytoplasmatic compartments which fuse the endocytic vesicles for redistribution of extracellular compounds to alternate destinations. EEA1 mediates the regulatory effects of 3'-phosphoinositides on membrane trafficking through its FYVE-type zinc finger domain.
Catalog #E2211-05
ApplicationsSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionELISA: 1:50
Western Blot (HeLa): 1:500
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa392-1411, TPSSKKPVRVCDACFNDLQG of human EEA1.
SpecificityRecognizes human EEA1
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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