Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Stem Cell Research » Anti -EED (embryonic ectoderm development, hEED, HEED, Polycomb protein EED, WAIT1, WAIT-1, WD protein associating with integrin cytoplasmic tails 1)

Anti -EED (embryonic ectoderm development, hEED, HEED, Polycomb protein EED, WAIT1,
WAIT-1, WD protein associating with integrin cytoplasmic tails 1)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.
Catalog #E2211-50D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000
Optimal dilutions to be determined by the researcher.
Positive ControlA431 Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2ak
Clone No9H228
HostMouse
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in 0.1 M Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant fusion protein of full length murine EED tagged with MBP.
SpecificityRecognizes mouse EED (5-6 isoforms of EED are detected). Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links