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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)

Anti -EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC IF
EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF (1,2). Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2,3).
Catalog #E2215-30B
ApplicationsSuitable for use in Western Blot, Immunofluorescence, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunofluorescence (IF-IC): 1:800
Immunohistochemistry (Paraffin): 1:100
Immunoprecipitation: 1:50
Chromatin Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG
Clone No9j16
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to the amino-terminal sequence of human EGR1.
SpecificityRecognizes endogenous levels of total human EGR1 at ~75kD. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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