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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)

Anti -EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IH
Early Growth Response 1, (EGR1) belongs to the EGR family of zinc finger transcription factors and regulates the expression of several tumor suppressors. Its expression is induced by diverse signals that initiate growth and differentiation. EGR1 suppresses or activates cell growth depending on the tumor type. Within the region that includes its three zinc finger domains, human EGR1 shares 99% aa sequence identity with mouse and rat EGR1.
Catalog #E2215-31
ApplicationsSuitable for use in Immunohistochemistry and ELISA. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 8-25ug/ml using basic antigen retrieval detected EGR1 in paraffin-embedded human prostate cancer tissue sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7H231
HostRat
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa282-433 from human EGR1expressed in E. coli (P18146)
SpecificityRecognizes human EGR1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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