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You are here:Home » Antibodies » Abs to Elastase » Anti -Elastase (Biotin)

Anti -Elastase (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IH
Catalog #E2230-20A
ApplicationsSuitable for use in Western Blot, ELISA, Immunohistochemistry, Immunomicroscopy. Other applications not tested.
Recommended DilutionELISA: 1:4000 to 1:20,000 (use 1ug of Elastase using peroxide-conjugated streptavadin and ABTS as a substrate for 30 minutes at room temperature)
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio1020 BAC molecules per Rabbit IgG molecule
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourcePorcine
Concentration~10mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% sodium azide, 40% glycerol. Labeled with biotin.
PurityPuri ed from monospeci c antiserum by delipidation, fractionation and ion exchange chromatography.
ImmunogenElastase [Porcine Pancreas]
SpecificityRecognizes porcine elastase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti biotin, anti rabbit serum as well as purified and partially purified Elastase (porcine pancreas).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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