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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -eIF2C1 (Eukaryotic Translation Initiation Factor 2C1, Argonaute 1, AGO1, Golgi Endoplasmic Reticulum Protein 95kD, GERP95, Q99, Putative RNA-binding Protein Q99)

Anti -eIF2C1 (Eukaryotic Translation Initiation Factor 2C1, Argonaute 1, AGO1, Golgi
Endoplasmic Reticulum Protein 95kD, GERP95, Q99, Putative RNA-binding Protein Q99)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4) and Piwi-interacting RNAs (piRNAs) (5), have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation and stress response (1,2). MicroRNAs regulate gene expression by modulating mRNA translation or stability (2). MicroRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of microRNA-mediated gene silencing such as repression of translation and mRNA turnover (1).
Catalog #E2237-25B
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by Protein A and peptide affinity chromatography.
ImmunogenSynthetic peptide corresponding to residues surrounding Lys400 of human Argonaute 1 protein.
SpecificityRecognizes endogenous levels of total Argonaute 1 protein. Species crossreactivity: Mouse, rat and monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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