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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -EIF2C1 (Protein Argonaute-1, Argonaute1, hAgo1, Eukaryotic Translation Initiation Factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding Protein Q99, AGO1)

Anti -EIF2C1 (Protein Argonaute-1, Argonaute1, hAgo1, Eukaryotic Translation Initiation
Factor 2C 1, eIF-2C 1, eIF2C 1, Putative RNA-binding Protein Q99, AGO1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
Catalog #E2237-25E
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:16,000
Western Blot: 0.3-1ug/ml, in transfected HEK293 on ~110, 35kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-KNASYNLDPYIQEF, from human EIF2C1, at the internal region of the protein (NP_036331.1).
SpecificityRecognizes human EIF2C1. Species sequence homology: Canine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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