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Specifications

The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5).
Catalog #	E2240-20
Molecular Weight	40kD nonphosphorylated, 50kD phosphorylated
Nonphosphorylated Elk1 fusion protein	Bacterially expressed GST-Elk1 fusion protein serves as a negative control. Supplied in SDS Sample Buffer. Store at -20°C.
Phosphorylated Elk1 fusion protein	GST-Elk1 phosphorylated by the Erk2 enzyme serves as a positive control. Supplied in SDS Sample Buffer. Store at -20°C.
Directions for Use	As controls, we recommend using 15ul of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading. 150ul wil be sufficient for 10 blots.
Western Blots	For controls, we recommend using 15ul of phosphorylated and nonphosphorylated Elk1 control extracts. Boil sample prior to loading.
Form	Supplied as a liquid in SDS Sample Buffer: 62.5mM Tris-HCl, pH 6.8 at 25°C, 2% w/v SDS, 10% glycerol, 50mM DTT, 0.01% w/v phenol red.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.