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You are here:Home » Biologicals » Biologicals-Cells-Embryonic Fibroblasts » Embryonic Fibroblasts, P2, Untreated, Mouse (CF-1 MEF)

Embryonic Fibroblasts, P2, Untreated, Mouse (CF-1 MEF)


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Mouse embryonic fibroblast cells. Passage #2, untreated, ~1x10e6 cells/vial.
All cell lines are produced under aseptic conditions. Each lot is tested to be free from bacterial/fungal, mouse pathogen and mycoplasma contamination before shipping.
Catalog #E2256-10
Cell TypeMouse Embryonic fibroblasts
Analysis Parameters
CriterionViability Test: > 60%
Sterility Test: No growth
Mouse Pathogen Test: Negative
Mycoplasma: Negative
Mouse ESC Support:
1. Viability is defined as the ratio of number of live cells over total number of cells after thawing. 2. Immersion method is used to inoculate directly into Tryptic Soy Broth media and Fluid Thioglycollate media to check for bacteria and fungal contamination. 3. PCR based assays for mouse pathogens including Sendai virus, mouse hepatitis virus, pneumonia virus, minute virus, parvovirus (MPV1,MPV2, MPV3) and Theilerís murine encephalomyelitis virus. 4. PCR based assay. 5. Mouse embryonic stem cells are plated and observed for growth on a layer of feeder cells by ES cell colony morphology.
StorageVapor phase of liquid nitrogen freezer.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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