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Specifications

Clone Host Grade Applications Polyclonal Rabbit B IP

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and Aand B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to -catenin and inhibits its nuclear accumulation (8). Emerin is known to interact with nuclear lamins, barrier-to-autointegration factor (BAF), nesprin-1alpha , and a transcription repressor. Recent studies demonstrated that Emerin is required for HIV-1 infectivity (9). It is speculated that patients suffering from Emery-Dreifuss muscular dystrophy may have immunity to or show an irregular infection pattern to HIV-1. Mutations in Emerin are a major cause of X-linked recessive Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10).
Catalog #	E2257-01
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Immunoprecipitation: 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Purity	Affinity chromatography.
Immunogen	Synthetic peptide (KLH- coupled) corresponding to residues surrounding Ala212 of human Emerin.
Specificity	Detects endogenous levels of total Emerin protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.