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Anti -EMP3 (Epithelial Membrane Protein 3)
Anti -EMP3 (Epithelial Membrane Protein 3)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
FC |
|
| Epithelial membrane protein 3 (EMP3) is a multipass transmembrane protein in the peripheral myelin protein 22 family. It is expressed as a 20-25kD molecule depending on the degree of glycosylation. EMP3 interacts with the P2X7 purinergic receptor on monocytes. Its dysregulation is associated with glioma and neuroblastoma. Human EMP3 shares 93% aa sequence identity with mouse and rat EMP3. | | | Catalog # | E2261 | | Application | Suitable for use in Flow Cytometry, Other applications not tested. | | Recommended Dilution | Flow Cytometry: This antibody was validated for flow cytometry using human PBMC. For intracellular staining to detect EMP3, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25ug/ml and add 10ul of the diluted solution to 1-5 x 10e5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome. | | Optimal dilutions should be determined by each laboratory for each application. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 8B16 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 0.2ml sterile 40-50% glycerol, PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | NS0 cells transfected with human EMP3. | | Specificity | Recognizes human EMP3 cell transfectants but not irrelevant transfectants. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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