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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -EMP3 (Epithelial Membrane Protein 3)

Anti -EMP3 (Epithelial Membrane Protein 3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Epithelial membrane protein 3 (EMP3) is a multipass transmembrane protein in the peripheral myelin protein 22 family. It is expressed as a 20-25kD molecule depending on the degree of glycosylation. EMP3 interacts with the P2X7 purinergic receptor on monocytes. Its dysregulation is associated with glioma and neuroblastoma. Human EMP3 shares 93% aa sequence identity with mouse and rat EMP3.
Catalog #E2261
ApplicationSuitable for use in Flow Cytometry, Other applications not tested.
Recommended DilutionFlow Cytometry: This antibody was validated for flow cytometry using human PBMC. For intracellular staining to detect EMP3, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25ug/ml and add 10ul of the diluted solution to 1-5 x 10e5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as anti-mouse IgG conjugated to a fluorochrome.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No8B16
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 0.2ml sterile 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0 cells transfected with human EMP3.
SpecificityRecognizes human EMP3 cell transfectants but not irrelevant transfectants.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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