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You are here:Home » Antibodies » Abs to Transcription Factors, Homeobox (HOX) » Anti -EN2 (Homeobox Protein Engrailed-2, Homeobox Protein en-2, Hu-En-2)

Anti -EN2 (Homeobox Protein Engrailed-2, Homeobox Protein en-2, Hu-En-2)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
EN2 is thought to have a role in controlling development. In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. This protein has been implicated in the control of pattern formation during development of the central nervous system.
Catalog #E2270-11
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:4000
Western Blot: 0.1-0.3ug/ml, observed in mouse and rat brain lysates on ~30kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-NHSTTAKEGKSDSE, from rat EN2, at C-terminal (NP_001418.2).
SpecificityRecognizes rat EN2. Species sequence homology: Canine, human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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